Isolation and determination of indigenous endophytic bacteria from greenhouse tomato plants

Abstract

Rhizosphere bacteria represent the largest part of the rhizosphere microbial community. basic knowledge including isolation, identification and characterization of the bacteria is very important in order to utilize the beneficial of rhizobacteria as plant growth and health promoting. Endophytic bacteria, bacteria that reside within living internal plant tissue, may be considered as a plant growth and plant health promoting rizobacteria. In this experiment, indigenous endophytic bacteria from tomato plants in the greenhouse were isolated and identified. The isolation of bacteria was based on the trituration and surface sterilization method. The bacteria were identified using Fatty Acid Analysis (FAME) and Gas Chromatography (GC).