Please use this identifier to cite or link to this item: http://repository.ipb.ac.id/handle/123456789/54682
Title: Isolasi Senyawa Fenolik Aktif sebagai Antioksidan dari Daun Dandang Gendis (Clinachantus nutans)
Isolation of Phenolic Active Compounds as Antioxidant from Dandang Gendis (Clinachantus nutans) Leaves.
Authors: Tohir,Dudi
Hanif, Novriyandi
Soekma, Indah
Keywords: Bogor Agricultural University (IPB)
Issue Date: 2012
Abstract: The leaves of dandang gendis (Clinacanthus nutans) is one of the medicinal plants that have potency as antioxidant. The purpose of this study is to isolate some bioactive compounds from the plant which belongs to Acantaceae family to obtain the active component of phenolic compounds with high antioxidant activity. The antioxidant activity was determined by radical scavenging assay using 1,1-diphenyl-1-picrylhydrazyl radical. Fractionation using preparative thin layer chromatography with eluent of n-butanol:water:acetic acid (4:5:1) resulted three fractions. The most active fraction was the fraction 2 with 50% inhibition concentration (IC50) value of 89.50 mg L-1, while the IC50 for a control compound, which was butylated hydroxy toluene (BHT) was 6.87 mg L-1. These results indicated that the antioxidant activity of fraction 2 was much lower than that of BHT. Phenolic compounds test was performed by gas chromatography‒mass spectrometry revealed three suspected phenolic compounds, namely p-isopropenylphenol, o-hydroxyacetophenone, and p-hydroxyacetophenone.
Daun dandang gendis (Clinacanthus nutans) merupakan salah satu tanaman obat yang berpotensi sebagai antioksidan. Tujuan penelitian ini ialah mengisolasi senyawa bioaktif dari tanaman yang termasuk famili Acantaceae untuk mendapatkan komponen aktif senyawa fenolik dengan aktivitas antioksidan yang tinggi. Aktivitas antioksidan ditentukan dengan metode penggaitan radikal bebas 1,1-difenil-2-pikrilhidrazil. Dari hasil fraksinasi menggunakan kromatografi lapis tipis preparatif dengan eluen n-butanol:air:asam asetat (4:5:1) diperoleh 3 fraksi. Fraksi teraktif ialah fraksi 2 dengan nilai konsentrasi hambat 50% (IC50) 89.50 mg L-1, sedangkan IC50 untuk senyawa kontrol, hidroksi toluena terbutilasi (BHT) sebesar 6.87 mg L-1. Hasil ini menyimpulkan bahwa aktivitas antioksidan fraksi 2 jauh lebih rendah bila dibandingkan dengan aktivitas BHT. Uji senyawa fenolik dilakukan dengan kromatografi gas‒spektrometer massa dan diduga terdapat 3 senyawa fenolik, yaitu p-isopropenilfenol, o-hidroksiasetofenon, dan p-hidroksiasetofenon.
URI: http://repository.ipb.ac.id/handle/123456789/54682
Appears in Collections:UT - Chemistry

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