Pengaruh metode aplikasi bakteri endofit terhadap perkembangan nematoda peluka akar (Pratylenchus brachyurus) pada tanaman nilam

Abstract

Endophytic bacteria is one of the important agents recently used for controlling plant parasitic nematodes. P. brachyurus is one of the factors affecting the productivity of patchouli (Pogostemon cablin Benth.) in Indonesia. The objectives of the research were to find out an efficient application method of endophytic bacteria to reduce P. brachyurus on patchouli. The research was conducted in the Nematology Laboratory, Departement of Plant Protection, Bogor Agricultural University and in the Laboratory and Greenhouse of Indonesian Spice and Medicinal Crops Research Institute, from January to July 2005. The research used randomized complete design with two factors, the first factor was application method (drencing and deeping), the second factor was bacteria isolates (NJ2, NJ25, NJ41, NJ46, NJ57, NA22, ERB21, ES32, E26).The results showed that the population of nematode was affected by the interaction between bacterial isolates and application method. While shoot weight, root length and plant height were affected by bacterial isolates. Bacillus NA22, Bacillus NJ46 and Bacillus NJ2 applicated by deeping the root into bacterial suspension significantly gave good result in reducing P. brachyurus, i.e. 75%, 63% and 60%. All bacterial isolates increased shoot weight, root length.