The effectivity of CR1aa medium on in vitro maturation, fertilization and early embryo development of goat oocyte

Abstract

The aim of present study was to compare different media for supporting in vitro maturation, fertilization and early embryo development of goat oocyte. Two kinds of media, namely TCM199 and CR1aaa media were used for in vitro maturation and embryos culture. Three different media, BO, TALP and CR1aaa were used for in vitro fertilization. Oocytes were maturated in TCM 199 or CR1aa media, respectively, for 24 hours at 38.5 derajat celsius in 5% CO2 incubator. In vitro fertilization was done in 5% CO2, incubator at 38.5% derajat Celsius using fresh ejaculated sperm. After 8 hours of insemination, zygotes were cultured in two kinds of culture media, namely TCM-199 and CR1aa media, respectively in 5% CO2 incubator up to day-5. The result showed that not significant difference in the precentsges of oocytes reaching metaphase II in the two maturation media. The fertilization rate in CR1aaa medium (63.2%) was significantly higher (P<0.05) than BO and TALP medium (48.9% and 50.0%, respectively). The cleavage rate and embryos development in CR1aa medium were 49.76% and 39.63%, they were significant (P<0.05) than those in TCM 199 medium (40.84% and 29.58%).