Sperm immobilization prior to intracytoplasmic sperm injection (ICSI) and oocyte activation improves early development of microfertilized goat oocytes

Abstract

A preliminary goat oocyte activation and intracytoplasmic sperm injection was evaluated in this study.Immature goat oocytes were cultured in TCM-199 supplemented with 10% goat serum for 27 hours for maturation at 38.50C in an atmosphere of 5% C0 in humidified air. In vitro matured oocytes were used for activation and intracytoplasmic sperm injection. In the first experiments, calcium ionophore were used at final concentration 0, 1, 50, 100 and 200 πM to evaluate sham-injected goat oocyte activation In the second experiment, the effect of immobilized of goat sperm cell on the cleavage and the development of sperm-injected goat oocyte was evaluated. Results indicated that in vitro matured goat oocyte did not activate spontaneously. Exogenous stimuli such as Calcium ionophore A23187 at certain level (in this experiment >50 πM) can be used to activate in vitro matured goat oocytes.In this study, results indicated that sperm injected as well as sham injected goat oocyte can initiate first several cycles of cleavage after activation with calcium ionophore. Results in the current study also indicated that immobilization of goat sperm cells increase the percentage of early embryo development to cleavage after intracytoplasmic sperm injection.