Perbandingan Metode dan Verifikasi Analisis Total Karbohidrat dengan Metode Luff-Schoorl dan Anthrone Sulfat

Abstract

Carbohydrate plays crucial role in food industry. Therefore an accurate, direct and reliable carbohydrate analysis is needed. Among many colorimetric methods for carbohydrate determination, the Anthrone-sulfuric acid is the most commonly used. The Anthrone-sulfuric method for carbohydrate analysis is simple and sensitive. However, the SNI official method for carbohydrate analysis employing the Luff-Schoorl method which is time consuming, difficult for untrained staff and the reduction reactions are seldom stoichiometric. Therefore a new candidate method employing Anthrone sulfuric acid was proposed to replace the SNI 01-2891-1992 total carbohydrate analysis. In this research both methods were compared using three matrices which represent general food matrices in liquid form based on AOAC proposed triangle scheme. Samples from the low, medium and high content of carbohydrate from the triangle scheme were selected. The selected samples were coconut milk, soy sauce and sweet soy sauce. Based on the comparation result, Anthrone method as a new proposed method proved ineligible to replace the SNI 01-2891-1992. Thus the next step taken was to verify the SNI 01-2891-1992 method through its repeatability and accuracy. Accuracy was accessed using reference material and standard addition. The repeatability showed acceptable precision. But the standard addition exhibited poor recovery value in SNI 01-2891-1992 method of total carbohydrate