Perkembangan in vitro dan in vivo embrio mencit tanpa zona pelusida

Abstract

The aim of this study was to examine the in vitro and in vivo development of zona pellucida•free mouse embryos transferred to the recipient. Embryos were flushed on 4 days of gestation (vaginal plug=1 day of gestation) and pellucida zona was removed by using 0.25% pronase for 4-5 min. Embryo were cultured in tissue culture medium (TCM) 199 supplemented with 0.4% bovine serum albumin (BSA) in 5% C02 incubator at 37°C for 5 h. There was no difference between zona pellucida-free blastocysts developed into expanded blastosysts (76%, 31/41 and 73%, 43/59 for zona pellucida-free blastocysts, respectively) in contras, zona pellucida intact morula developed to blastocysts better than zona pellucida morula (79%, 49/62 and 19%, 10/52 for zona pellucida-intact and zona pellucida-free morula, respectively) The transfer of zona pellucida intact blastocysts resulted in completely implantation (48%, 12/25 and 13%, 6/45 for zona pellucida-free blastocysts respectively), All animal (100%, 3/3) receved zona pellucida intact blastocysts got in pregnancy at the 10th days of gestation (67%, 4/6). These results demonstrate that zona pellucida-free embryos could develop both in vitro and in vivo.