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Title: | Viability Of Frozen-Thawed Bovine Ivm/Ivf Embryos In Relation To Aging Using Various Cryoprotectants |
Authors: | Boediono, Arief T. Otoi M. Yamamoto Saha, S. Suzuki, Tatsuyuki |
Issue Date: | 1994 |
Publisher: | IPB (Bogor Agricultural University) |
Abstract: | Bovine IVF embryos developed on Days 7, 8 and 9 were equilibrated with 1. 6 M propylene glycol (PG) , 1. 8 M ethylene glycol (EG) , 1. 1 M diethylene glycol (DEG) or 1 . 3 M ethylene glycol monomethyl ether (EME) for 10 to 20 min in modified phosphate buffered saline (mPBS) supplemented with 10% superovulated cow serum. The embryos were loaded into 0. 25-ml plastic straws and were placed directly into a O"C alcohol bath chamber and held for 2 min. They were cooled from O"C to -5.5"C at 1"C /min and then seeded, followed by a 10-min holding period at - 5. 5"C . The straws were then cooled to -30"C at 0. 3"C /min before plunging into liquid nitrogen. Embryos were thawed and placed directly into the culture medium and washed 3 times. The survival rates of the Day-9 embryos based on reappearance of blastocoele, expansion, and hatching after 48 h of post-thaw culture were significantly lower (P<0.01) than those of the Day-7 and 8 embryos, in all of the cryoprotectants tested. On the other hand, while the reappearance of blastocoele and expansion of blastocysts after 48 h of post-thaw culture were not significantly different among each cryoprotectant, the percentage of hatching blastocysts were significantly different between DEG and EME (P<0.05) 1 between DEG and EG (P<0.01) and between PG and EG (P<0.05). These findings demonstrate that the age of the embryo (Day 7 and 8) is very important for the successful freezing of IVF bovine embryos. Also, as to the hatching rates, EME and EG are superior as cryoprotectants than the other 2 cryoprotectants tested. |
URI: | http://repository.ipb.ac.id/handle/123456789/53236 |
Appears in Collections: | Anatomy, Phisiology and Pharmacology |
Files in This Item:
File | Description | Size | Format | |
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Therio1994-41-915-FrozenAging.pdf | Publication | 319.1 kB | Adobe PDF | View/Open |
ABO_Viability of frozen thawed_Abstract.doc | Abstract | 26.5 kB | Microsoft Word | View/Open |
ABO_Viability of frozen thawed_abstract_.pdf | Abstract | 39.54 kB | Adobe PDF | View/Open |
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