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Title: | The effect of sperm-oocyte incubation time on in vitro embryo development using sperm from a tetraparental chimeric bull |
Authors: | Sumantri, Cece Boediono, Arief Ooe, Masato Murakami, M. Saha, S. Suzuki, Tatsuyuki |
Issue Date: | 1997 |
Publisher: | IPB (Bogor Agricultural University) |
Abstract: | The present study was designed as 5 X 4 factorial to investigate the effects of using sperm from 5 bulls, and varied sperm-oocyte incubation times (5, 10, 15 and 20 h) on the fertilization, cleavage rates and blastocyst formation on an in vitro bovine embryo production system. The bulls included a tetraparental Chimera, its sires (Japanese Black and Limousin), its maternal grand-sires (Japanese Brown and Holstein). The proportion of polyspermy, 2-pronuclei formation, fertilization, cleavage and development to blastocyst were affected ( p < 0.01) by the duration of sperm-oocyte incubation, as well as by the interaction between bulls and their corresponding sperm-oocyte incubation time. Blastocyst rate observed after 5 h in oocytes inseminated with Chimera, Japanese Black and Limousin were higher ( p < 0.05) than those observed at 20 h incubation. The proportion of blastocysts from oocytes inseminated with Japanese Black observed at I 0 h of incubation did not differ from that of Chimera, but both were higher ( p < 0.05) than those observed for the Limousin, Japanese Brown and Holstein sires. The present study showed that there was an effect by the duration of sperm-oocyte incubation on in vitro embryo development. The optimal time of sperm-oocyte incubation for the Chimera was similar to that of its sires (Japanese Black and Limousin) but differed from its maternal grand-sires (Japanese Brown and Holstein). The fertilization rates for the sperm from the Holstein bull increased up to 15 h suggesting that this might be the only bull that would benefit from a long incubation period for insemination. © 1997 Elsevier Science B.V. |
URI: | http://repository.ipb.ac.id/handle/123456789/53210 |
Appears in Collections: | Anatomy, Phisiology and Pharmacology |
Files in This Item:
File | Description | Size | Format | |
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ARS1997-48-187-SpermIncubation.pdf | Publication | 414.32 kB | Adobe PDF | View/Open |
The effect of sperm-oocyte incubation time on in vitro embryo.pdf | Full text | 361.73 kB | Adobe PDF | View/Open |
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