Pemanfaatan Virus Simbion Dari Beberapa Spesies Parasitoid Untuk Meningkatkan Kinerja Eriborus Argenteopilosus (Cameron)

Abstract

Polydnaviruses (PDV) are present in the female reproductive tissues of parasitoid Ichneumonids and Braconids. PDV symbionts were extracted from the ovaries of the parasitoid Apanteles spp. and host larval tissues of Spodoptera litura (Fabricius) attacked by parasitoid Snellenius manilae Ashmead and identified using the negative staining methods. The ultrasructure appearance of PDV derived from the calyx of parasitoid Diadegma semiclausum Helen (Family: Ichneumonidae) tissues are determined by the presence of nucleocapsid elliptical surrounded by double bilayer membranes. PDV derived from parasitoid S manilae (Family: Braconidae) have nucleocapsid that are cylindrical, individually or in groups surrounded by single bilayer membranes. The extraction and centrifugation of ovary parasitoid and infected host larval tissues by parasitoid obtained through the sucrose gradient procedure produced PDV sediment originated from four spesies such as Eriborus argenteopilosus (Cameron) and D. semiclausum (Family: Ichneumonidae) as well as S. manilae and Apanteles spp. (Family: Braconidae). Immunological methods that followed showed that each antigens (Ag PDV) are compatible against their antibodies (Abs) were produced.