Rancang bangun proses fermentasi aseton-butanol-etanol dari hidrolisat tandan kosong kelapa sawit (elaeis guineensis JACQ)

Abstract

The delignification of empty fruit bunches of oil palm (EFB) may be hydrolyzed to major soluble sugars, such as glucose and xylose, which then can serve as feed stocks for a variety of fermentations producing value added products. Azemi et al. (1994) have proven that the hydrolyzate of solid palm waste was a good substrate ior the production of glutamic acid and single cell protein. This study reports the process studies of acetone-butanol-ethanol fermentation from empty fruit bunches of palm oil The EFB was delignifiedby NaOH 1 N for two hours, followed by autoclaving at 121 °C for 15 min saccbarification of the holocellulose was carried out in stirred tank bioreactor at 48 JC for 72 h, using citric buffer 0-5 M, 2% of cellulose and 0 4% cellobiase. Fermentation was studied anaerobicallly using three different levels of initial sugar content (40. 60, 80 g/l) and Clostridium acetobutylicum P 262 at 34°C without agitation The best result (14 23 g ABE/I) was obtained on the substrate of 60 g/l, giving yield of 27,2%, based on the sugar being consumed. The maximum specific growth rate of the bacteria was 0.287/h the specific production rate of ABE was 0.263 g ABE/g biomass., and the residual acid content on broth was 13 73 g/l.