Pengaruh pemberian bubuk ekstrak Cu-turunan klorofil daun cincau (Premna oblongifolia Merr.) terhadap profil lipid darah kelinci

Abstract

The aim of this research was to produce cincau copper-derivative chlorophyll powder and to observe its physico-chemical properties and the effect on blood lipid profile of 5 month male New Zealand White rabbits. The blood lipid profile observed were total cholesterol, triglyceride, HDL (high-density lipoprotein) cholesterol, and LDL (low-density lipoprotein) cholesterol. A two months treatment was conducted. A random block design was applied with the following treatment on each experimental unit: P0 (basal diet, negative control), PI (basal diet with cholesterol 0.1% (w/w) added, positive control), P2 (basal diet with cholesterol 0.1% (w/w) and 16.7 mg/kg-body weight/d natural chlorophyll powder added), P3 (basal diet with cholesterol 0.1% (w/w) and 16.7 mg/kg-body weight/d copper-derivative of chlorophyll powder added), P4 (basal diet with cholesterol 0.1% (w/w) and 50.1 mg/kg-body weight/d copper-derivative of chlorophyll powder added), P5 (basal diet with cholesterol 0.1% (w/w), and 16.7 mg/kg-body weight/d commercial chlorophyll powder added. The results of study showed that cincau leaves chlorophyll extract solution with Cu2+ 100 mg/L level produce the cincau copper-derivate chlorophyll powder with highest pH, solubility, and greenness compared to other copper levels. Feeding with copper-derivative of chlorophyll cincau powder (P3) most likely decreased total cholesterol, triglycerides, LDL cholesterol increased the HDL cholesterol, and decreased atherosclerosis risk after 2 month as compare to feeding contain of natural chlorophyll (P2).