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Title: | Pengembangan metode elisa untuk mendeteksi keberadaan koproantigen fasciola gigantica pada ruminansia: model uji diagnostik untuk human fasciolosis |
Authors: | Samarang |
Keywords: | Bogor Agricultural University (IPB) Fasciola gigantica Fasciolosis Coproantigen IgY Sandwich ELISA |
Issue Date: | 2011 |
Publisher: | IPB (Bogor Agricultural University) |
Abstract: | The study was designed to develop ELISA method to detect coproantigen of Fasciola gigantica in ruminant as a model for human fasciolosis diagnostic test. Fasciolosis Diagnostic Test. Under direction of FADJAR SATRIJA and SRI MURTINI. Excretory Secretory (ES) antigen were isolated from adult stage of F. gigantica incubated in the RPMI medium at 370C for 4 hours. Each worm produced roughly 0.18 mg ES antigen. ES protein showed five protein bands with the molecular weight of 104.99, 90.05, 56.43, 47, 30.27 kDa in SDS-PAGE analysis. P olyclonal antibodies against ES antigen were raised from rabbits and chicken egg yolk. These polyclonal antibodies were used in sandwich ELISA for detection of coproantigen using IgY as capture antibody and IgG as detection antibody. Specificity and sensitivity test revealed that the ELISA have 87,5% sensitivity and 100% specificity compared to gold standard post-mortem examination. |
URI: | http://repository.ipb.ac.id/handle/123456789/51620 |
Appears in Collections: | MT - Veterinary Science |
Files in This Item:
File | Description | Size | Format | |
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2011sam.pdf Restricted Access | Full Text | 794.39 kB | Adobe PDF | View/Open |
Abstract 2011sam-2.pdf Restricted Access | Abstract | 316.31 kB | Adobe PDF | View/Open |
Cover 2011sam.pdf Restricted Access | Cover | 305.76 kB | Adobe PDF | View/Open |
Bab I Pendahuluan 2011sam-3.pdf Restricted Access | Bab I | 316.8 kB | Adobe PDF | View/Open |
Bab II Tipus 2011sam-4.pdf Restricted Access | Bab II | 437.24 kB | Adobe PDF | View/Open |
Bab III Metode 2011sam-5.pdf Restricted Access | Bab III | 351.06 kB | Adobe PDF | View/Open |
Bab IV Hasil 2011sam-6.pdf Restricted Access | Bab IV | 517.31 kB | Adobe PDF | View/Open |
Bab V Simpulan 2011sam-7.pdf Restricted Access | Bab V | 303.09 kB | Adobe PDF | View/Open |
Daftar Pustaka 2011sam-8.pdf Restricted Access | Daftar Pustaka | 365.21 kB | Adobe PDF | View/Open |
Lampiran 2011sam-9.pdf Restricted Access | Lampiran | 397.64 kB | Adobe PDF | View/Open |
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