Penghambatan oksidasi LDL dan akumulasi kolesterol pada makrofag oleh ekstrak temulawak (Curcuma xanthorriza Roxb)

Abstract

Coronary heart disease is caused among others by atherosclerosis, which is the result ol oxidized tow density lipoprotein (LDL) and cholesterol accumulation on the macrophage. This were reported to be inhibited by temulawak (Curcuma xanthonzza Roxb). The objective ot this study was to find out the types and consentretions of temulawak extract which could inhibit LDL oxidation, and to find out the effect of temulawak extract on the accumulation of cholesterol on macrophage. Temulawak was extracted by water, ethanol, aceton and dichlorometane. inhibition of LDL oxidation was analyzed by measuring the level of melonaldehyde content of the oxidized LDL-CuSOi which were given water extract, ethanol extract, aceton extract and dichtorometane extract of temulawak at concentrations of 43 jjg, 430 /jg, and 4300 fig per ml of LDL. The percentage of malonaldehyde reduction due to addition of water, ethanol, acetone and dichloromethane extract were 44.27; 47.68; 51.83 and 61.2 respectively. The inhibition of LDL oxidation by temulawak extract depends on the concentrations. The percentage of malonaldehyde reduction due to addition of temulawak extract ol 43 pg, 430 jjg, and 4300 per ml of LDL were 43.83; 5672; and 53.89 Concentrations of temulawak extract resulting in the highest inhibition of LDL oxidation was 430 pgM LDL Temulawak extract tends to inhibit cholesterol accumulation on the macrophage There is a correlation between the inhibition of cholesterol accumulation on the macrophage and the inhibition of LDL oxidation by temulawak extract