Protein Concentration Of Excretory/Secretory Released by Ascaridia galli Larvae

Abstract

Ascaridia galli adutt worm were collected in the intestine of village chickens from the traditionally market of Bogor. The eggs in uteri of female adult worms were expelled, prepared and colected under stereomicroscope. The eggs were incubated in sterile aquadestilata at room temperature 21 - 30 days to developed embrionated eggs (L2). A. galli larvae (L3) recovered from laying hen intestines 7 days after each oesophagus inoculation with 6000 L2 were cultured (5-10 ml-1) in flasks containing RPMI 1640 media, pH 6.8, without phenol red and suplemented with 100 units ml-1 penicillin G, 100 ug ml-1 streptomycin, 5 pg ml-1 gentamycin, and 0.25 gg ml-1 kanamycin. Cultures were incubated at 37°C in 5% C02 and excretorylsecretory product of U. released in culture was collected after 3 days. The amount of concentration was determined from the absorbance at 595 nm. The result showed that protein concentration of excretory /secretory product released by L3 of A. galli were 9.83 ppm. The result indicate that the small amount of protein concentration was determined, but it is generally assummed that the protein released by L3 of A. galli play an important role in host immune response mechanisms