Please use this identifier to cite or link to this item:
http://repository.ipb.ac.id/handle/123456789/50997
Title: | Association study and expression analysis of porcine ESR1 as a candidate gene for boar fertility and sperm quality |
Authors: | Gunawan, Asep Kaewmala, Kanokwan Uddin, Muhammad Jasim Cinar, Mehmet Ulas Tesfaye, Dawit Phatsara, Chirawath Tholen, Ernst Looft, Christian Schellander, Karl |
Issue Date: | 2011 |
Publisher: | IPB (Bogor Agricultural University) |
Abstract: | Male fertility is impaired through the lack of ESR1 (Estrogen Receptor 1) but little is known about the ESR1 roles in boar spermatogenesis and fertility. Therefore, this research was aimed at investigating the association with sperm quality and boar fertility traits in a total of 300 boars both from purebred Pietrain and Pietrain × Hampshire crosses. A SNP in coding region of ESR1 g.672C > T in exon 1 was associated with sperm motility (P < 0.05) and plasma droplet rate (P < 0.01) while the polymorphism in non-coding region of ESR1 g.35756T > C in inton 1 was associated with non-return rate (P < 0.05). Furthermore, to analyse the mRNA and protein expression of ESR1 in boar reproductive tissues, a total of six boars were divided into two groups [Group I (G-I) and Group II (G-II)], where G-I had relatively better sperm quality. ESR1 expression was higher in tissues collected from G-I boars than those of collected from G-II boars, and the difference in mRNA expression was significant (P < 0.01) in head of epididymis. The ESR1 protein expression results from western blot coincided with the results of qRT-PCR. The ESR1 protein localization observed a strong staining in the cytoplasm of Sertoli cell in the testis, in the epithelial cells in head and tail of epididymis, in smooth muscle in tail of epididymis, and in the post acrosomal region and tail of the spermatozoa. These results will improve the understanding of the functions of the ESR1 in spermatogenesis within the reproductive tract and will shed light on ESR1 as a candidate in the selection of boar with good sperm quality and fertility. |
URI: | http://repository.ipb.ac.id/handle/123456789/50997 |
Appears in Collections: | Animal Production Technology |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Gunawan et al 2011in Anim Reprod Sci.pdf | Full Text | 792.63 kB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.