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http://repository.ipb.ac.id/handle/123456789/47532
Title: | Isolasi dan purifikasi inhibitor RNA helikase virus hepatitis C dari bakteriosin bakteri asam laktat S34 |
Authors: | Suryani Mustopa, A. Zaenal Putri, Skotia Fitriastri |
Keywords: | Bogor Agricultural University (IPB) |
Issue Date: | 2011 |
Publisher: | IPB (Bogor Agricultural University) |
Abstract: | Target molecular therapy is one of method to prevent hepatitis C virus which was developed recently. NS3 Helicase/RNA helicase hepatitis C virus inhibition using inhibitory peptide are target in molecular therapy. Bacteriocin are inhibitory peptides which can inhibit hepatitis C virus through inhibition of NS3 helicase. Bacteriocin is a peptide which is produced by lactic acid bacteria. The aim of this research were to isolate and to purify bacteriocin from S34 lactic acid bacteria which has high activity to inhibit RNA helicase of hepatitis C virus. S34 lactic acid bacteria were identified using 16S RNA amplification and DNA sequencing identification of S34 lactic acid bacteria was Lactobacillus plantarum WCFS1. The bacterial growth reached logaritmic phase at 7 hours and stationary phase at 10 hours. Bacteriocin were isolated and purified by ammonium sulphate precipitation with 80% saturation, gel filtration chromatography, and characterization of bacteriocin through molecular weight analysis and measured protein concentration. Bacteriocin from S34 lactic acid bacteria were included in 2nd class bacteriocin, had 64.20 % of inhibition against RNA helicase hepatitis C virus, had molecular weight about 1.7-4.2 kDa, and specific activity in the amount of 2.84 x 106 U/mg. |
URI: | http://repository.ipb.ac.id/handle/123456789/47532 |
Appears in Collections: | UT - Biochemistry |
Files in This Item:
File | Description | Size | Format | |
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Abstract_ G11sfp.pdf Restricted Access | Abstract | 360.5 kB | Adobe PDF | View/Open |
BAB I Pendahuluan_ G11sfp.pdf Restricted Access | BAB I | 361.82 kB | Adobe PDF | View/Open |
BAB II Tinjauan Pustaka_ G11sfp.pdf Restricted Access | BAB II | 814.62 kB | Adobe PDF | View/Open |
BAB III Bahan dan Metode_ G11sfp.pdf Restricted Access | BAB III | 396.49 kB | Adobe PDF | View/Open |
BAB IV Hasil dan Pembahasan_ G11sfp.pdf Restricted Access | BAB IV | 2.08 MB | Adobe PDF | View/Open |
BAB V Simpulan_ G11sfp.pdf Restricted Access | BAB V | 361.93 kB | Adobe PDF | View/Open |
Cover_ G11sfp.pdf Restricted Access | Cover | 358.68 kB | Adobe PDF | View/Open |
Daftar Pustaka_ G11sfp.pdf Restricted Access | Daftar Pustaka | 366.2 kB | Adobe PDF | View/Open |
G11sfp.pdf Restricted Access | Full Text | 2.75 MB | Adobe PDF | View/Open |
Lampiran_ G11sfp.pdf Restricted Access | Lampiran | 492.1 kB | Adobe PDF | View/Open |
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