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http://repository.ipb.ac.id/handle/123456789/47464
Title: | Karakterisasi gen nifD bakteri metanotrof asal sawah |
Authors: | Rusmana, Iman Akhdiya, Alina Rachmawati, Resti |
Keywords: | Bogor Agricultural University (IPB) Methanotrophic bacteria nitrogen fixation nitrogenase nifD |
Issue Date: | 2011 |
Publisher: | IPB (Bogor Agricultural University) |
Abstract: | Methanotrophic bacteria are methane oxidizing bacteria. Some methanotrophs able of fix nitrogen using nitrogenase enzyme. Nitrogenase is composed of two multisubunit metallo protein. Component I contains the active site to nitrogen reduction, encoded by the nifD and nifK genes. Methanotrophic isolates of BGM 3 and BGM 9 had high ammonium accumulation cultured in Nitrate Mineral Salts (NMS) free nitrogen medium. nifD gene fragmens from BGM 3 and BGM 9 were amplified and sequenced. Sequences analyzed using bioinformatics tools such as Blast-N, Blast-X, Expasy Translate Tools, Conserved Domain (CDD) and Scan PROSITE. Phylogenetic construction made using MEGA 4 software. Sequence analysis showed that the nifD sequences of BGM 3 and BGM 9 most closely related to nifD fragmen of Xanthobacter autotropicus PY2. The Identity values of nifD sequences was 92% and 91% respectively. nifD genes from these isolates encode FeMo protein, which was 4Fe4S and 2Fe2S clusters. The nifD gene of BGM 3 and BGM 9 suspected to have same ancestor with the nifD gene of Xanthobacter autotropicus PY2 and Bradyhizobium japonicum USDA 110. |
URI: | http://repository.ipb.ac.id/handle/123456789/47464 |
Appears in Collections: | UT - Biology |
Files in This Item:
File | Description | Size | Format | |
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G11rra.pdf Restricted Access | fulltext | 1.06 MB | Adobe PDF | View/Open |
G11rra_Cover.pdf Restricted Access | Cover | 293.01 kB | Adobe PDF | View/Open |
G11rra_Abstract.pdf Restricted Access | Abstract | 293.69 kB | Adobe PDF | View/Open |
G11rra_BAB I Pendahuluan.pdf Restricted Access | BAB I | 302.78 kB | Adobe PDF | View/Open |
G11rra_BAB II Bahan dan Metode.pdf Restricted Access | BAB II | 322.78 kB | Adobe PDF | View/Open |
G11rra_BAB III Hasil dan Pembahasan.pdf Restricted Access | BAB III | 971.73 kB | Adobe PDF | View/Open |
G11rra_Kesimpulan & Daftar Pustaka.pdf Restricted Access | Kesimpulan | 294.23 kB | Adobe PDF | View/Open |
G11rra_Lampiran.pdf Restricted Access | Lampiran | 325.5 kB | Adobe PDF | View/Open |
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