Production Of Cyclodextrin Glycosyl Transferase From Local Strain Bacteria (Bacillus pumilus BK1) Using Tapioca As Carbon Source

Abstract

Cyclodextrin glycosyl transferase or CGT-ase (α-1, 4-glucan-4-glycosil transferase, E.C.2.4.1.19) is an enzyme which catalyzes degradation of starch into cyclic oligosaccharides called cyclodextrins. Known cyclodextrins consist of 6, 7 and 8 glucose units linked through a-1, 4 glycoside. This experiment was to study the possibility of producing CGT-ase using local strain bacteria that can utilize tapioca as carbon source. The bacteria used in this experiment were local strain of Bacillus pumilus BK1 and B. macerans IFO 3490 as a reference. The effectiveness of tapioca as carbon source was compared with soluble starch. CGT-ase activity was measured using spectrophotometric and chromatographic (HPLC) method. During fermentation, total bacteria was counted by means of total plate count method. The fermentation was carried out in rotary shaking incubator and stirred tank reactor. The result showed that B. pumillus BK1 is more capable in using tapioca as carbon source than that of B. macerans IFO 3490. The growth of B. pumillus BK1 in soluble starch and tapioca media was better than that of B. macerans IFO 3490. The colony of B. pumillus BK1 reached 14.6 - 21.2 x 107 using soluble starch and in tapioca media respectively