Vitrifikasi ovarium mencit menggunakan etilen glikol dan dmso sebagai krioprotektan dan viabilitasnya pasca autotransplantasi di subkapsula ginjal

Abstract

The aim of this study was to examine viability of vitrified mouse ovary using ethylene glycol (EG) and dimethyl sufoxide (DMSO) as cryoprotectant. Thirty two female DDY mouse (8 weeks of age) were divided into four groups : control (fresh ovaries), 30% EG, 30% DMSO, and 15% EG +15% DMSO. Modified phosphate buffered saline cantaining 20% fetal bovine serum, 0.5M sucrose and cryoprotectant were used as vitrification solution. Ovaries were exposed with solution contained 10% and 30% cryoprotectant 5 minutes of each at room temperature, loaded in 0.5 ml straws and vitrified in liquid nitrogen (1960C). After warming in water at 37° C, ovaries were autografted under the kidney capsule. Mouse were eximaned oestrous cycles daily and on day-30, mouse were sacrificed and ovaries were fixed for histology. Viability of ovaries were 100%, 13%, 0%, and 19% in control, 30% EG, 30% DMSO, and 15% EG +15% DMSO groups, respectively. Oestrous cycles were detected in 100%, 13%, 0%, and 13% mice in control, 30% EG, 30% DMSO, and 15% EG + 15% DMSO groups, respectively. Histology examination of viable ovaries showed follicles development. This result showed that ovaries vitrified in solution contained 30% EG and 15% EG + 15% DMSO as cryoprotectant could be survived after autografted under the kidney capsule.