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Title: | Development of Ovine Embryos Derived from Oocytes Matured in vitro in the Absence of C02 |
Other Titles: | Development of Ovine Embryos Derived from Oocytes Matured in vitro in the Absence of C02 |
Authors: | Margawati, E.T. Supriatna, Iman Yusuf, Tuty Laswardi Supriondho, Y. |
Issue Date: | 1997 |
Publisher: | IPB (Bogor Agricultural University) |
Abstract: | The purpose of the study was to determine the effect of C02 during In Vitro Maturation (IVM) and subsequent development into morula/blastocyst stages in sheep and to develop a method of in vitro maturation in ruminant animals. Oocytes from abattoir ovaries were colected by methods of aspiration and spraying of media using a^-18-G needle with aspiration medium of hepes-199 (H199) + 0.4% BSA + 50 i^g/ml Heparin. The oocytes were divided into 3 groups and treated separately as follows: T1) oocytes were cultured in an eppendorf containing maturation medium (Bicarbonate-199 + 10% FCS + 10|ig/ml FSH + 10 jag/ml hCG +1 i^g/ml Estradiol) overlaid with mineral oil. The IVM medium was equilibrated in 5% C02 incubator prior to culture of the oocytes then matured in the absence of C02; T2) oocytes were; T3) oocytes were cultured in a petri dish containing maturation drops (as T1 medium) overlaid with mineral oil it was equilibrated in 5% C02 incubator prior to culture then matures in 5% C02 incubator. All cultures were maintained at 38°C in a humaditified incubator for 24 hours. The mature oocytes were fertilized in BO (Brackett and Oliphant) medium with concentration of approximately 12 x 106 sperm/ml for 6 hours and cultured in systhetic oviductal fluit (SOF) supplemented with amino acids (AA) and bovine serum albumin (BSA) at 38°C in humaditified incubator with 5%C02 for 7 days. The result showed that there was a highly significant effect of C02 during oocytes maturation on the percentage of cleaved oocytes (P<0.01). However, oocytes matured in the absence of C02 did not affect on either the percentages of morula/blastocyst or the rates of morula/blastocyst. This study suggests that ovine embryos derived from oocytes matured in vitro in the absence of C02 can be developed in vitro morula or blastocyst stages. |
URI: | http://repository.ipb.ac.id/handle/123456789/45183 |
Appears in Collections: | Faculty of Veterinary |
Files in This Item:
File | Description | Size | Format | |
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Development of Ovine Embryos Derived from Oocytes Matured_Full text.pdf | Full Text | 505.28 kB | Adobe PDF | ![]() View/Open |
Development of Ovine Embryos Derived from Oocytes Matured_Abstract.pdf | Abstract | 26.97 kB | Adobe PDF | ![]() View/Open |
Development of Ovine Embryos Derived from Oocytes Matured_Abstract.doc | Abstract | 27 kB | Microsoft Word | View/Open |
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