Efek Sinbiotik Prebiotik Asal Pangan Lokal dengan Enterococcus Faecium Is 27526 Terhadap Bal dan Berat Badan Pada Tikus Percobaan (Synbiotic Effect of Prebiotic Local Food and Probiotic Enterococcus faecium IS 27526 to viable fecal lactic acid bacteria and Body Weight of Mice)

Abstract

Protein Energy Malnutrition problem in children younger than five can be over come by supplementation functional weaning food utilizing local food which can stimulate the immune response. According to Nutrition Information Centre of the University of Stellenbosch (NICUS), synbiotic effect of probiotic and prebiotic is potential in improving health through improvement of survival and availability of gut microbiota in the instestine, however further in vivo research is needed before supplemented to young children. The aim of this study is to validate the synbiotic effect of functional biscuit of local food based (arawroot and sweet potato powder) and probiotic Enterococcus faecium IS-27526 on viable fecal lactic acid bacteria and bodyweight of mices. The in vivo experiment was carried out in Animal Experiment Laboratory, Puslitbang Gizi (PPPG), Bogor. Fecal microbiota of mices were carried out at the laboratory of Microbiology, Fateta IPB. There were 7 groups of mice (5 each): Al = ransum; A2 = ransum + biscuit with arrawroot powder + probiotic cream; A3 = ransum + biscuit with sweet potato powder + probiotic cream; A4 = ransum + biscuit with arrawroot powder + probiotic cream + FOS; A5 = ransum + biscuit with sweet potato powder + probiotik cream + FOS; A6 = ransum + probiotic cream; A7 = ransum + probiotic cream + FOS, were randomized and treated for 3 weeks supplementation . The results show that after 3 weeks supplementation, there was a significant different on the body weight of functional biscuit with ararrot plus FOS and E. faecium IS-27526 probiotic cream, followed by functional biscuit with ararrot and probiotic cream, 86,8 g (151,48%) from initial body weight, and 80,8 g (138,59%), respectively, as compared to other group of treatments. Viable fecal lactic acid bacteria of mices was increased after supplementation with functional biscuit with ararrot plus FOS and E. faecium IS-27526 probiotic cream, significantly (p< 0,05), followed by functional biscuit with arawrrot and probiotic cream