Propagation and Formulation of Chitinolytic Bacteria on Organic Waste for Bio-Pesticide

Abstract

The objectives of this research were to get some bacterial isolates (P. fluorescens and Bacillus sp.) indicating chitinolitic and antagonistic to fungal pathogen and to get the composition modification of organic waste apropriately for growing of selected isolates. From 83 bacterial isolates evaluated, 21 and 32 of them were chitinolitic P. fluorescens and Bacillus spp respectively. Not all isolates indicating chitinolitic were antagonistic bacteria to fungal plat pathogen, only 12 and 10 isolates of P. fluorescens and Bacillus spp respectively were potential to inhlhit the grow of fungal pathogen (Rizoctonia solani, Fusarium oxisporum, Helminthosporium maydis, dan Qricularia oryzae). Two selected isolates that the most potent chitinolitic and antagonistic were able to grow on the modificated organic waste as well as grow on Luria Broth mediuq with the population densities achieved lo9 cfu/ml (log 9,50) and lo8 cfu/rnl (log 8,75) for P. fluorescens and Bacillus spp., respectively.