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Title: | The Potency of Dextranase from Arthrobacter sp. Strain B7 as Dental Plaque Removal |
Other Titles: | HAYATI Journal of Biosciences Vol. 12 No.4 Tahun 2005 |
Authors: | Baktir, Afaf Zaini, Noor Cholies Murdiyatmo, Untung Kuntaman |
Issue Date: | 2005 |
Publisher: | IPB (Bogor Agricultural University) |
Series/Report no.: | Vol 12;No 4 |
Abstract: | Dextranase of Arthrobacter sp. strain B7 (B7DEX enzyme) was characterized in this study. This enzyme hydrolyzed sucrose and dextran, but not other glucans (starch, nigeran, cellulose, β-soluble glucan). It also hydrolyzed glucan from dental plaque with the activity of 7.38 + 0.66 U/ml, where the activity toward dextran was 31.88 + 1.24 U/ml. The enzyme exhibited the pH optimum of 7 and the temperature optimum of 50 oC. Its optimum stability was at pH 7 and 50 oC. The enzyme was inhibited by Fe3+, Cu2+, Zn2+, and Ag+, but not by the anionic detergent (SDS) and the nonionic detergent (Triton-X). The enzyme was activated by Ca2+, Na+, Mg2+, and saliva. |
URI: | http://repository.ipb.ac.id/handle/123456789/43387 |
ISSN: | 0854-8587 |
Appears in Collections: | Hayati Journal of Biosciences |
Files in This Item:
File | Description | Size | Format | |
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Afaf Baktir.pdf | e-Journal | 96.7 kB | Adobe PDF | View/Open |
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