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http://repository.ipb.ac.id/handle/123456789/43281
Title: | Allotopic expression of a gene encoding FLAG Tagged-subunit 8 of yeast mitochondrial ATP synthase |
Other Titles: | HAYATI Journal of Biosciences Vol. 13 No.1 Tahun 2006 |
Authors: | Artika, I Made |
Issue Date: | 2006 |
Publisher: | IPB (Bogor Agricultural University) |
Series/Report no.: | Vol 13;No 1 |
Abstract: | Subunit 8 of yeast mitochondrial ATP synthase is a polypeptide of 48 amino acids encoded by the mitochondrial ATP8 gene. A nuclear version of subunit 8 gene has been designed to encode FLAG tagged-subunit 8 fused with a mitochondrial signal peptide. The gene has been cloned into a yeast expression vector and then expressed in a yeast strain lacking endogenous subunit 8. Results showed that the gene was successfully expressed and the synthesized FLAG taggedsubunit 8 protein was imported into mitochondria. Following import, the FLAG tagged-subunit 8 protein assembled into functional mitochondrial ATP synthase complex. Furthermore, the subunit 8 protein could be detected using anti-FLAG tag monoclonal antibody. |
URI: | http://repository.ipb.ac.id/handle/123456789/43281 |
ISSN: | 0854-8587 |
Appears in Collections: | Hayati Journal of Biosciences |
Files in This Item:
File | Description | Size | Format | |
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I Made Artika.pdf | e-Journal | 65.92 kB | Adobe PDF | View/Open |
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