Please use this identifier to cite or link to this item: http://repository.ipb.ac.id/handle/123456789/42761
Title: Isolation, Characterization, and Genetic Diversity of Ice Nucleation Active Bacteria on Various Plants
Other Titles: HAYATI Journal of Biosciences Vol. 16 No. 2 Tahun 2009
Authors: Waturangi, Diana Elizabeth
Tjhen, Amelia
Issue Date: 2009
Publisher: IPB (Bogor Agricultural University)
Series/Report no.: Vol 16;No 2
Abstract: Ice nucleation active (INA) bacteria is a group of bacteria with the ability to catalyze the ice formation at temperature above -10 oC and causing frost injury in plants. Since, most of the literature on INA bacteria were from subtropical area, studies of INA bacteria from tropical area are needed. We sampled eight fruits and 36 leaves of 21 plant species, and then identified through biochemical and genetic analysis. INA bacteria were characterized for INA protein classification, pH stability, and optimization of heat endurance. We discovered 15 INA bacteria from seven plants species. Most of bacteria are oxidase and H2S negative, catalase and citrate positive, gram negative, and cocoid formed. These INA bacteria were classified in to three classes based on their freezing temperature. Most of the isolates were active in heat and pH stability assay. Some isolates were analysed for 16S rRNA gene. We observed that isolates from Morinda citrifolia shared 97% similiarity with Pseudomonas sp. Isolate from Piper betle shared 93% similarity with P. pseudoalcaligenes. Isolate from Carica papaya shared 94% similarity with Pseudomonas sp. While isolate from Fragaria vesca shared 90% similarity with Sphingomonas sp.
URI: http://repository.ipb.ac.id/handle/123456789/42761
ISSN: 1978-3019
Appears in Collections:Hayati Journal of Biosciences

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