Clooning of araA Gene Encoding L-Arabinose Isomerase from Marine Geobacillus stearothermophillus Isolated from Tanjung Api, Poso, Indonesia

Please use this identifier to cite or link to this item: http://repository.ipb.ac.id/handle/123456789/42707

Title:	Clooning of araA Gene Encoding L-Arabinose Isomerase from Marine Geobacillus stearothermophillus Isolated from Tanjung Api, Poso, Indonesia
Other Titles:	HAYATI Journal of Biosciences Vol. 17 No. 1 Tahun 2010
Authors:	Fitriani, Dewi Saksono, Budi
Issue Date:	2010
Publisher:	IPB (Bogor Agricultural University)
Series/Report no.:	Vol 17;No 2
Abstract:	L-arabinose isomerase is an enzyme converting D-galactose to D-tagatose. D-tagatose is a potential sweetenersucrose substitute which has low calorie. This research was to clone and sequence araA gene from marine bacterial strain Geobacillus stearothermophilus isolated from Tanjung Api Poso Indonesia. The amplified araA gene consisted of 1494 bp nucleotides encoding 497 amino acids. DNA alignment analysis showed that the gene had high homology with that of G. stearothermophilus T6. The enzyme had optimum activity at high temperature and alkalin condition.
URI:	http://repository.ipb.ac.id/handle/123456789/42707
Appears in Collections:	Hayati Journal of Biosciences

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