Injeksi Spermatozoa Domba Hasil Pengeringbekuan ke dalam Sel Telur Menggunakan Teknik Intracytoplasmic Sperm Iw'ection (IC SI)

Abstract

In ths experiment, the ability offeeeze-dried ram spermatozoa to decondense and to support pronucleus formation after injection into oocyte using ICSI method was evaluated. Aceto lacmoid staining was used to assess decondensation and pronucleus formation following ICSI.' Results of these experiments revealed that freeze-dried spermatozoa had the ability to decondense (2%) and to induce IPN formation (34%) following injection into oocytes but fail to form 2PN. However, 30% of 2PN and 40% oocytes were obtained when oocytes were injected with fresh spermatozoa. In conclusion, freeze-dried ram spermatozoa were able to decondense and to support 1PN formation after ICSI. Key words: spermatozoa, freeze-dried, oocyte, ICSI, decondensation, pronucleus.