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Title: | Transformasi Genetik Tanaman Kentang cv. Atlantik Dengan Mengintroduksikan Gen Hordothionin untuk Mendapatkan Ketahanan terhadap Penyakit Bakteri |
Other Titles: | Buletin Agronomi Vol.31 No.2, Th. 2003 |
Authors: | Nurhasanah Wattimena, G.A. Purwito, Agus Wiendi, Ni Made Armini Suharsono |
Issue Date: | 2003 |
Publisher: | IPB (Bogor Agricultural University) |
Series/Report no.: | Vol.31 No.2 |
Abstract: | Hordothionins are small anti-bacteria proteins present in barley endosperm. To reveal the potential of this proteins for engineering bacterial disease resistance into potato, a semi-synthetic hordothionin gene construct was introduced in potato cv. Atlantic via Anrobacterium tumefaciens strain LBA 4404, under the control of a caul~jlower mosaic virus (CaMV) 35s promotor. The in vitro grown stem (internodus) was used in this research. Afer 6 weeks in regeneration medium and 2 weeks in rooting medium there were 22 regenerated plants that were screened in kanamycine containing medium. PCR analysis using spesrfic primer from CaMV 35Spromotor showed the presence of ampl19ed T-DNA in 4 transgenic lines from 22 putative transgenic plants were tested. The in vitro toxicity against Ralstonia solanacearum tested from transgenic lines showed variation in resistance level. There were only 2 of the transgenic lines were tolerant, while one of them was moderate tolerant even one of them was susceptible. |
URI: | http://repository.ipb.ac.id/handle/123456789/35467 |
Appears in Collections: | Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) |
Files in This Item:
File | Description | Size | Format | |
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2.5.pdf | e-jurnal_full Text | 802.12 kB | Adobe PDF | View/Open |
2.5_abstract.pdf | e-jurnal_abstract | 68.77 kB | Adobe PDF | View/Open |
2.5_abstract.ps | PostScript | 163.78 kB | Postscript | View/Open |
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