Perakitan Kultivar Kentang Unggul Indonesia secara Cepat dengan Metode Turunan Klonal Biji Tunggal daD Pra - Evaluasi Secara In Vitro

Abstract

At least ten years needed to abtain new potato cultivar through sexual hybridization, somatic hybridization or through genetic transformation,T o short cut this process,L aboratory of Biotechnology,D epartmento f Agronomy,I PB employeda strategys o called single seedi n vitro clonal decent( SSICD)b y Usings electedp arental linesf or TPS( True Potato Seed) production. This breeding consist of in vitro pre evaluation for resistance wilt, fusarium wilt, black leg, rot knot nematode and maturity. Using the same number of bacterial cel/ (lif cel/lml), there were positive correlation between in vitro test for disease resistance through dripping test or dripping test with greenhouse test through direct inoculation of Ralstonia solanancearum. Resistant clones to fusarium wilt and verticil/ium were also resistant to bacterial wilt. In vitro tuberization could be use to evaluate maturity of potato cultivar.