Penyediaan Bibit Pisang Tanduk (Musa paradisiaca L. AAB Grup) secara Kultur Jaringan

Abstract

The micropropagation of plantain cv. Pisang randuk to supply seedlings was done. There were 3 in vitro experiments: (i) The eflects of Adenin sulfat (0; 100; 150; 200 ppm) - Le4 (0; 3; 6; 9ppm) on the plantlet production, (ii) The eflects of R4P (0; 3; 6; 9 ppm) - IAA (0; 2; 4; 6; 8 ppm) on the shoot multiplication and (iii) The efect of IR4 (2; 4; 6; 8 ppm) on the root formation of microshoot. The extra virrum experiment was done by potted planrlets on the compost medium and then transferred to soil medium. me combination of Adenin sulfur - IAA (experiment i) induced the production of plantlets, but the maximum amount was only 4 plantlets per culture. In experiment (ii), planrlets were produced in media without plant growth regulator and media containing IAA only (5 plantlets per culture). The addition of BAP induced multiple shoot (19 shoots/culture) in 2 months, but there was no root formarion. The addition of 1124 induced root.formation. Plantlets were successfilly to be acclimatitated and grew well become seedlings.