Isolation and Determination of Enzymatic Activity of Selected Fungi on Sugarcane Bagasse as Feed for Ruminant

Abstract

It was found that ester and covalent bond between lignin, polysaccharides, and protein could reduce the digestibility of cellulose and hemicelluloses of sugarcane bagasse. Objectives of this experiment were to identify and to isolate the fungi that capable of degrading the lignocellulosic materials in sugarcane bagasse and to determine the decomposition ability of enzymatic selected fungi. The method used to culture the fungi was enrichment and platting method, while the method used for selection of fungi was the enzymatic selection method. Isolated fungi in this experiment were: Aspergillus sp, Penicillium citrinum, Penicillium sp(2), Penicillium sp(3), Penicillium sp(4), Penicillium sp(5), Penicillium sp(6), Memnoniella sp(1), Memnoniella sp(2), dan Helminthosporium sp. Further test showed that these isolated fungi have cellulolytic activity.