Detection of Enterobacter sakazakii and other Enterobacter sp from Dairy Cow’s Milk in Boyolali and Sleman

Abstract

Enterobacter sakazakii is considered as an opportunistic pathogen that has been implicated in severe forms of necrotizing colitis and meningitis especially in neonates with a mortality rare varying from 40%-80%. The natural habitat E.sakazakii is not well understood and has been reported as frequency isolated from different environments including soil, rats, flies, milk powder factories, chocolate factories and households. A total of 100 samples were obtained from dairy cow’s milk were studied. The presence of E.sakazakii and Enterobacter sp was detected using the Holt et al., 1994; Guillaume et al., 2005 and Turner et al., 2000 procedure on TSBA medium. E.sakazakii was not isolated from both district Sleman and Boyolali. However, E.cloacae was found in 33 of 75 isolates (44%) of samples from Sleman. Meanwhile 12.7% E.cloacae and 5.4% E.gergoviae was found of samples from Boyolali.