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http://repository.ipb.ac.id/handle/123456789/32062
Title: | Cloning, DNA Sequence, and Expression of Aeromonas caviae WS7b Chitinase Gene |
Authors: | Malik, Amarila Wenuganen, S. Suwanto, Antonius Tjahjono, Budi |
Issue Date: | 2003 |
Publisher: | IPB (Bogor Agricultural University) |
Abstract: | A chitinase-producing bacterium, designated WS7b, was isolated from a soil sample obtained from a black-pepper plantation on Bangka Island, Indonesia. Fatty-acid methyl-ester analysis indicated that the isolate was Aeron~onas cnviae. A chitinase gene from WS7b was cloned in a pUC19-based plasmid vector, but without its natural promoter. The complete nucleotide sequence of the gene was determined, and the structural gene consisted of a 2748-bp region encoding 864 amino acids. DNA sequence analysis indicated that the gene had been cloned without its promoter, and this was confirmed by chitinase-plate assay of the truncated version of the gene in Escherichici coli. The chitinase gene product shgwed amino-acid sequencc similarity to chiA from A. caviae. Chitinase enzyme activity was determined spectrophotonietrically, using colloidal chitin azure as substrate for extracellular and intracellular fractions. The ability of the ,:.;:;.,:r cloned in E. coli to hydrolyze chitin was less than that of the enzyme in its indigenous host. Index Entries: Aero17zolzns caviae; chitinase gene; DNA sequence; chitinolytic activity. |
URI: | http://repository.ipb.ac.id/handle/123456789/32062 |
Appears in Collections: | Faculty of Mathematics and Natural Sciences |
Files in This Item:
File | Description | Size | Format | |
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Amarila Malik_Herni (10 hal).pdf | Publication | 1.44 MB | Adobe PDF | View/Open |
Amarila Malik_Herni (10 hal).ps | Postscript | 2.05 MB | Postscript | View/Open |
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