Aktivitas antioksidan minyak bekatul padi awet dan fraksinya secara In vitro

Abstract

Some researches indicated that oryzanol had antioxidant activity, however, the information about the oryzanol role in prevention of low density lipoprotein (LDL) and human lymphocyte from oxidantion under oxidative stress was still limited The objective of this study was to investigate the antioxidant activity of oryzanol at concentrations based on rice bran beverage model in preventing LDL and lymphocyte from oxidation Human plasma were supplemented with the samples of nice bran 011 [RBO), unsaponifiable matter and oryzanol IR-64, oryzanol IR-64 3x and oryzanol standard at the concentrations of 308 3, 22 2, 5 2, 10 4 and 10 4 pgimi, respectively Afterward, the human LDL were collected by ultracentnfuge and d~lutedu nbl a concentration of 200 pg protein/mlw as reached Human LDL isolates were then oxidlzed with CuS04 5 /iM for measuring antioxidant activity of the samples The length of incubation, H202 concentration, penod of sample supplemented Into human lymphocyte culture were determined before the antioxidant activity of RBO and its fraction in lymphocyte was measured The samples used in the lymphocyte were RBO IR-64, unsapon~f~abmlea tter 1/3-64, and oryzanol standard at the concentrations of 133 2 - 2,132 0 ~rglml,9 6 - 153 6 lig/ml, and 2 4 - 37 7,ug/rnl, consecutively The result showed that malonaldehyde concentration m human LDL decreased significantly (~s= 0 05), 15 - 41°0 and 39 - 56 % compared to the control The absorbance of 11v1ngly mphocyte cell ln culture was not influenced by the type and concentration of RE0 and its fracbon The addition of hydrogen peroxide (Hz04 3 mM into culture significantly lowered the absorbance as compared to culture without H2O2.