Profil DNA Genom Xanthomonas campestris dengan Menggunakan Schizotyping

Abstract

Scbizotyping was employed to reveal unique fingerprints of particular strains or pathovala of Xanthmonas campestris (Pamawl) Dowson. Digestion of X. campestris pv. cumpeslris gnomic DNA with each of these restriction endonucleases: Dml, Asel, Spel, Xbal, or SnaBl generated 15-40 rrsolvabb frrrgmemts witb molecular size ranging from approximately 15 to 589 kilo base pairs (kb). 00 tbc other bad, Swl, Pacl, Pmel, and I-Ceul were found to digest X. campestris pv. campest& gaomk DNA verg iofquentty (kss tban 15 fragments) which will be useful for sizing and pbyskal mapping of the genome. DNA-banding patterns, generated by Asel- w Spel- restriction endonuclerw digestion of total gcnomk DNA, were able to distingubb unambiguously strains of X. cmpestris pv. glycims (Nakmo) Dyefrom tbat of X. campestris pv. campestris and among the strains within X. campestris pv. glycines. ThLs metbod bas promise for Identifying various strains or pathovare within tbe genus Xunthomonm wbicb is extremely important in understanding the epidemiology as well as the molecular basis of patbogeaicity of bacterial-pustule disease.