Induksi Kalus dan Regenerasi Dioscorea composita HemsI

Abstract

Callus induction and planlet regeneration of D. composira Hemsl are described. Nodal cuttings and young sctds of D. conrpodfa wert induced to produce callus and planlets on modified MS medium, tJmt are supplemented with various conclentrations of p w t b rg(&tors and 0.8% agar. In the culture of nodal segments, 8 single plantlet was produced by the proliferation of axiilnry buds and was not from the undifferentiated callus. However, from seed cultures, anhayogenic d u s was able to be qpcratcd to plantlets after subculturing into regeneration mcdiurn.