Analisis fenotipe dan kemotaksonomi rhizobia yang diisolasi dari Aeschynomene spp

Abstract

Eleven strains isolated from nodules ofAeschynomene spp. were subjected to phenotypic characterization such as biological, morphological, and physiological characters and chemotaxonomic study. The result showed that four bacterial groups were identified as: (i) Photosynthetic rhizobia which contains 3 isolates that produce photosynthetic pigments under lightdark cycle in aerobic condition and have 16:0,18:0, and 18:l as major fatty acid; (ii) non-photosynthetic Bradyrhizobia have 16:O and 18:l as major fatty acid; (iii) Rhodopseudomonaspalustris group which consists of 3 isolates that grow and produce photosynthetic pigment anaerobically in the presence of light and have 16:0,16:1,18:0, and 18:l as major fatty acid; and (iv) an isolate that produce photosynthetic pigment anaerobically in the presence of light and has 16:0, 16:1, and 18:l as major fatty acid. These data suggest that the first group is photosynthetic rhizobia, the second group is non-photosynthetic rhizobia, the third and fourth group are anaerobic photosynthetic bacteria.