Seleksi dari Kalus yang Menghasilkan Antosianin Tinggi pada Polygonum Tinchtorium AIT

Abstract

The effectsof kind and concentration of plant growth regulators on anthocyanin synthesis of six cultivars of Pol-iurn tinchtorfurn Ait were studied. The cultivar tested were (1) Shoujyacko Shirobana, (2) Matsuae Ai, (3) Hyaklcnn, (4) Shatyouko Akabana, (5) Miyagi Ai and (6) Akaguki Kosenbon. Among those cultivars, cultivar no. l, 2 and 3 did not synthesize anthocyanin, cultivar no. 4 produced slightly anthocyanin while cultivar no. 5 and 6 produced large amount of anthocyanin. The best condition to induce anthocyanin formation was kinetin (KIN)-indob acetic acid (IAA) (KIN 10 ppm; IAA 0, 5,1,3,5,10 ppm) under light condition using petiole as an explant. The amount of anthocyanin produced by selected calks was four times higher than unscreened callus. Indican was a little detected on MeOH extract from green callus