Analisis enzim alanin amino transferase (alat), aspartat amino transferase (asat), urea darah, dan histopatologis hati dan ginjal tikus putih galur sprague-dawley setelah pemberian angkak

Abstract

Acute toxicity of angkak had been tested on 2 months aged male Sprague-Dawley white rats. Twenty five rats were divided into 5 groups; control, 2.5 g/kg body weight (bw), 5 g/kg bw, 10 g/kg bw and 15 g/kg bw, and each group was administered by angkak in water orally. The toxic effect of angkak to liver and kidney were tested by biochemical analysis for the activity of enzyme alanin amino transferase (ALAT/ EC 2.6.1.2), enzyme aspartate amino transferase (ASAT/ EC 2.6.1.1) and the level of urea in blood at one day before (H-1) and after (H+1) the treatment, as well as 6 days after the treatment (H+6). The mortality rate and clinical symptoms were observed after 24 hours until 6 days after treatment. The rats were necropsied to observe the lesion of liver and kidney both macroscopically and microscopically.