Keragaman Genetik Sapi Peranakan Ongole (Po) Berdasarkan Uji Dna Mikrosatelit

Abstract

Recently, microsatellite DNA is often used as a marker for studying the genetic variability. DNA microsatellite is a tandemly repeated sequence abundantly present in the present eukario.tic genome. In the present study, six microsatellite loci were used to asses the level of genetic variability among populations of PO caHle. Heterozigosity value (E), average heterozigosity (~) and population genetic variation of four different subpopulation were calculated and compared. The genetic distances and the differences between subpopulation were calculated to understand the degree of gene differentiation among subpopulation. In addition, the fixation indices were estimated to understand the breeding structure of population or the pattern of selection associated with polymorphic alleles. The result showed that PO caHle population exhibited the high genotype variation and high heterozygosity. The average heterozigosity of four lIubpopulation were 0,505-0,606. DNA microsatellite of PO caHle il found polymorphic. Among population, there wall found any genetic differentiation (GST • 37,1%) because they were not cross breeding between lubpopulation (PST. 0,3841) and genetic distances were 0,242-0,767. Keyword: microsatellite.