Konstruksi Pustaka Genom Kedelai Kultivar Slamet

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DC Field	Value	Language
dc.contributor.author	Suharsono
dc.date.accessioned	2010-05-25T02:58:22Z
dc.date.available	2010-05-25T02:58:22Z
dc.date.issued	2002
dc.identifier.uri	http://repository.ipb.ac.id/handle/123456789/25240
dc.description.abstract	Genomic library of soybean cv. Slamet had been constructed in λ phage vector. Partial digestion of total DNA was conducted using 0.014 units Sau3AI per μg DNA at 370C for 30 minutes. By using 0.5 μg insert DNA and the molarity ratio between insert DNA:vector = 2.9:1, the titer of 5 x 105 plaque forming unit was obtained. Among them, 85% or 4.25 x 105 pfu are recombinant. Since the length of insert DNA in λ recombinant phages is 15 kb in average, and the genome size of diploid soybean is 2.23 x 106 kb, therefore, the genomic library constructed was expected to contain all genome of soybean cv. Slamet.	id
dc.publisher	IPB (Bogor Agricultural University)
dc.title	Konstruksi Pustaka Genom Kedelai Kultivar Slamet	id
dc.title.alternative	Construction of Genomic Library of Soybean Cultivar Slamet	id
Appears in Collections:	Biology

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File	Description	Size	Format
Suharsono _SS.pdf	Publikasi	272.89 kB	Adobe PDF	View/Open
Suharsono _SS.ps	Postscript	634.7 kB	Postscript	View/Open

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