Morphological Characteristics of in vitro Cultured Cells Derived from Tumor in Domestic Animals

Abstract

Twenty-two tumors derived from dog, cat, sheep, chicken, Guinea pig and cow were processed for cell culture. Cultured cells derived from mammary gland tumors could be grouped into three types; spindle, large elongated and polygonal shaped cells. The polygonal cells formed pavement-like colonies and blister-like structure or domes. The cultured cell from mixed tumor and adenoma complex showed various cell morphologies, while the cells derived from adenoma and adenocarcinoma revealed a monotonous appearance. Mesothelioma-derived cultured cells were seen as an attached cell with large and long cytoplasmic processes and the other was small to medium floating cells. The cultured cells from canine transmissible venereal tumor (CTVT) shown as floating and attached cells and cells from melanoma were elongated to spindle and round to oval shape cells with dark cytoplasmic granules. The cells derived from liposarcoma showed numerous cytoplasmic vacuoles, while the pattern of cells in monolayer derived from basal cell tumors, which could be grouped into three types revealed similarity with the histological appearance of the paraffin section. All these findings indicated that the tissue culture system might useful for the precise understanding of the morphology, proliferation and differentiation of some tumors in domestic animals.