Please use this identifier to cite or link to this item: http://repository.ipb.ac.id/handle/123456789/24855
Title: Isolat Strepcoccus agalactiae Asal Sapi Penderita Mastitis Subklinis : I. Ekspresi fenotip isolat inVitro
Other Titles: Media Veteriner, Tahun 1997, Vol. 4 No.1
Authors: Utama, Iwan H.
Rejeki, Ninuk S.
Sukada, I Made
Wibawan, I Wayan Teguh
Issue Date: 1997
Publisher: IPB (Bogor Agricultural University)
Series/Report no.: Vol. 4 No.1
Abstract: Thirty six isolates Streptococcus agalactiae from subclinical mastitis cattle in Bogor were esanlincd their phenotypic espressions such as hemolytic pattern. supernatant turbidity in liquid medium, and colony morphology in semisolid medium (agar semi solid). Nine, fifteen and ttvelve isolates showed their a, 0, and y hemolytic patterns respectively. Fourteen isolates showed turbid supernatant, 18 isolates with less turbid supernatant, and the rest gave clear supernatant in fluid medium. In agar semi solid, 15 isolates showed mostly thick diffuse colonies in combination with thin diffuse and compact colonies, 17 isolates with mostly thin diffuse colonies in combination with thick diffuse and compact colonies. The rest isolates showed compact without diffuse colonies. There was a relation between growth pattern in fluid medium and colony morphology in agar semi solid, and the variability of colony morphologies in agar semi solid indicated the incidence of phase variation.
Sebanyak 36 isolat lapang Streptococcus agalactiae asal sapi penderita mastitis subklinis di daerah Bogor diamati ekspresi fenotipnya yang meliputi tipe hemolisis yang dihasilkan, kekeruhan pada supernatan media cair dan bentuk koloni pada media semi padat (Soft agar). Sebanyak sembilan, 15 dan 12 isolat memperlihatkan hemolisis tipe a, P, clan y. Ekspresi fenotip pada supernatan medium cair memperlihatkan 14 isolat dengan supernatan media keruh, 18 isolat dengan supernatan media sedikit kemh dan sisanya dengan supernatan media jernih. Sedangkan pada media semi padat memperlihatkan 15 isolat dengan bentuk koloni kebanyakan dlfus tebal, difus tipis dan kompak, 17 isolat dengan koloni kebanyakan difus tipis, difus tebal dan kompak, sisanya dengan koloni kompak tanpa adanya koloni difus. Terlihat adanya hubungan antara poia pertumbuhan pada media cair dan bentuk koloni pada media semi padat, keragaman koloni pada agar semi padat menunjukkan adanya variasi fase.
URI: http://repository.ipb.ac.id/handle/123456789/24855
Appears in Collections:Faculty of Veterinary
Media Veteriner
Veterinary Clinic Reproduction and Pathology

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