Molecular Analysis of Intraspecifik Variations of the Indonesian Cochliobolus heterostrophus

Abstract

The primary objective of the current research was to detect genetic variations witllin tlie Indonesiali isolates of Cocliliobolcis hetcrostrophus this collected from ecologically different places of the country at molecular level using PCR-RFLP analyses. The ~wi~nepra ir of NS3 and NS6 produced atnplification fragnlent in all of tlie isolates tested. A single fragnlent of estinlated 907 bp \\,as observed in tlie PCR product pattern. RFLP analysis of tlie PCR product employing tliree restriction enzymes. IfaeIII, NlraI, and RsaI, respectively, did not reveal intraspecific variations within the fungus. Similarly, nucleotide sequences of portion of snlall subunit of tlie riboson~al DNA gene of two of the isolates collected showed no appreciable differences. illdic;tti~ig the absence of genetic diversities alnong the isolates tested. A phylogenetic tree wasMconstruc!ed and the Indoriesiarl C. Iceter~~c.irol~rhe~ptrse,s ented IIJ' SRI-1 isolate, \\as found to be ~)Iiylogenetica!lyl ocated near C. sativcn, a closely related s~,ecies.