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Title: | Pengaruh Serum Homolog Dan Heterolog Terhadap Proses Pematangan Dan Pembuahan Oosit Domba Di Dalam Medium Biakan In Vitro |
Other Titles: | The Influence of Homologous And Heterologous Sera In Culture Medium On In Vitro Maturation And Fertilization Of Sheep Oocytes |
Authors: | Djuwita, Ita Rusyiyantono, Yohan Muhamad, Kusdiantoro Purwantara, Bambang Sukra, Yuhara |
Issue Date: | 1998 |
Publisher: | Bogor Agricultural University (IPB) |
Abstract: | The influence of various types of sera i.e. Fetal Calf Serum (FCS), Bovine Post Estrous Serum (BPES) as heterolog sera; and Ewes Fetal Serum (EFS) and Ewes Serzrrn (ES) as homologue sera on in vitro maturation and fertilization of sheep oocytes was investigated s sing -l'iss~ie Culture Medium 1 991-M (TCM 199. Ciibco). Bovinc sera wcre collected I'rom cow at seben days post-estrus, while ewes sera were collected during estrous (ES-HO) and six days post estrous (ES-H6). The result shows that the maturation rate of oocytes cultured in medium supplemented with EFS, ES-HO and ES-H6 at concentrations of 32.9 %, 68.7 %, and 67.6 % respectively is better than those supplemented with FCS and BPES at concentrations of 22.2 % and 28.9 %, respectively. In vitro fertilization rate were significantly higher in medium supplemented with ES-HO and ES-H6 at concentration of 65.4 % and 65.8 % (P < 0.05) than that supplemented with BPES at concentration of 31.7 %. In conclusion, ewes sera could be used in culture medium for promoting both in vitro maturation and fertilization rate of sheep oocytes. Key words: ewes serum, in vitro maturation, in vitr |
URI: | http://repository.ipb.ac.id/handle/123456789/24506 |
Appears in Collections: | Anatomy, Phisiology and Pharmacology |
Files in This Item:
File | Description | Size | Format | |
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Ita Djuwita (Herni).pdf | Publikasi | 446.43 kB | Adobe PDF | View/Open |
Ita Djuwita (Herni).ps | Postscript | 780.94 kB | Postscript | View/Open |
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