Pengaruh Suplementasi Lactobacillus plantarum dan Mineral Organik terhadap Fermentasi Rumen dan Produksi Metana secara In Vitro

Abstract

Penelitian ini bertujuan untuk mengevaluasi pengaruh penambahan Lactobacillus plantarum (sebagai agen probiotik) yang dikombinasikan dengan mineral organik kromium(Cr), selenium (Se), dan seng (Zn) terhadap aktivitas fermentasi rumen secara in vitro. Rancangan percobaan yang digunakan adalah Rancangan Acak Kelompok (RAK) dengan15 perlakuan dan 5 ulangan, terdiri atas: Z0 = ransum basal (kontrol); Z1 = Z0 + L.plantarum; Z2 = Z0 + Zn; Z3 = Z0 + Se; Z4 = Z0 + Cr; Z5 = Z1 + Zn; Z6 = Z1 + Se; Z7 =Z1 + Cr; Z8 = Z1 + Zn + Se; Z9 = Z1 + Zn + Cr; Z10 = Z1 + Se + Cr; Z11 = Z1 + Zn + Se+ Cr; Z12 = Z0 + Zn + Se; Z13 = Z0 + Zn + Cr; dan Z14 = Z0 + Zn + Se + Cr. Parameter yang diamati meliputi pH rumen, produksi gas total, produksi gas metana, kecernaan bahan kering (KCBK), kecernaan bahan organik (KCBO), serta konsentrasi N-NH3. Data dianalisis menggunakan analysis of variance (ANOVA), apabila terdapat perbedaan nyata (P<0,05), dilanjutkan dengan uji kontras ortogonal. Hasil penelitian menunjukkan bahwa suplementasi L. plantarum tunggal maupun dalam kombinasi dengan mineral Zn, Se, dan Cr belum mampu secara optimal meningkatkan kecernaan dan konsentrasi N-NH3. Namun, perlakuan tersebut terbukti menurunkan produksi gas metana selama fermentasi.

This study aims to evaluate the addition of Lactobacillus plantarum (as a probioticagent) combined with organic minerals chromium (Cr), selenium (Se), and zinc (Zn) onrumen fermentation activity in vitro. The experimental design used was a Randomized Block Design (RBD) with 15 treatments and 5 replications, consisting of: Z0 = basal ration (control); Z1 = Z0 + L. plantarum; Z2 = Z0 + Zn; Z3 = Z0 + Se; Z4 = Z0 + Cr; Z5 = Z1 +Zn; Z6 = Z1 + Se; Z7 = Z1 + Cr; Z8 = Z1 + Zn + Se; Z9 = Z1 + Zn + Cr; Z10 = Z1 + Se +Cr; Z11 = Z1 + Zn + Se + Cr; Z12 = Z0 + Zn + Se; Z13 = Z0 + Zn + Cr; and Z14 = Z0 + Zn+ Se + Cr. The parameters observed included rumen pH, total gas production, methane gas production, dry matter digestibility (DMD), organic matter digestibility (OMD), and N-NH3 concentration. Data were analyzed using analysis of variance (ANOVA), and if there was a significant difference (P < 0.05), it was continued with an orthogonal contrast test. The results showed that supplementation of L. plantarum alone or in combination with the minerals Zn, Se, and Cr was not able to optimally increase N-NH3 digestibility and concentration. However, this treatment was proven to reduce methane gas production during fermentation.