Suplementasi Vitamin E dan Selenium (Se) dalam Pakan Mengandung Minyak Ikan Lemuru terhadap Profil Darah Ayam Broiler

Abstract

Penelitian ini bertujuan mengevaluasi pengaruh suplementasi vitamin E dan selenium dalam pakan mengandung minyak ikan lemuru terhadap profil darah ayam broiler. Penelitian menggunakan Rancangan Acak Lengkap (RAL) 4 perlakuan 5 ulangan, dengan setiap ulangan berisi 10 ekor ayam broiler. Pakan perlakuan yang digunakan yaitu P0 = ransum basal mengandung minyak ikan lemuru 3% (kontrol), P1 = P0 + 200 ppm vitamin E + 0,3 ppm selenium, P2 = P0 + 300 ppm vitamin E + 0,6 ppm selenium, P3 = P0 + 400 ppm vitamin E + 0,9 ppm selenium. Peubah yang diukur meliputi profil darah lengkap yaitu eritrosit, leukosit, hemoglobin, hematokrit, serta diferensiasi leukosit (limfosit, heterofil, eosinofil, monosit, basofil, dan rasio H/L). Hasil penelitian menunjukkan bahwa suplementasi perlakuan P3 (400 ppm vitamin E + 0,9 ppm Se) nyata (P<0,05) meningkatkan jumlah leukosit dan menurunkan basofil. Suplementasi perlakuan P1 dan P2 tidak memberikan pengaruh yang nyata (P>0,05) terhadap peubah yang diamati. Simpulan penelitian yaitu suplementasi vitamin E 400 ppm dan selenium 0,9 ppm meningkatkan respon imun ayam broiler yang ditandai dengan meningkatnya leukosit dan menurunnya basofil.

This study aimed to evaluate the effect of vitamin E and selenium supplementation in feed containing lemuru fish oil on the blood profile of broiler chickens. The study used a Completely Randomized Design (CRD) with 4 treatments and 5 replications, each replication consisting of 10 broiler chickens. The treatment feeds used were P0 = basal ration containing 3% lemuru fish oil (control), P1 = P0 + 200 ppm vitamin E + 0.3 ppm selenium, P2 = P0 + 300 ppm vitamin E + 0.6 ppm selenium, P3 = P0 + 400 ppm vitamin E + 0.9 ppm selenium. The variables measured included complete blood profiles, namely erythrocytes, leukocytes, hemoglobin, hematocrit, and leukocyte differentiation (lymphocytes, heterophils, eosinophils, monocytes, basophils, and H/L ratio). The results showed that supplementation of treatment P3 (400 ppm vitamin E + 0.9 ppm Se) significantly (P<0.05) increased the number of leukocytes and decreased basophils. Supplementation of treatments P1 and P2 did not have a significant effect (P>0.05) on the observed variables. The conclusion of the study is that supplementation of vitamin E 400 ppm and selenium 0.9 ppm increases the immune response of broiler chickens, which is characterized by an increase in leukocytes and a decrease in basophils.