Please use this identifier to cite or link to this item: http://repository.ipb.ac.id/handle/123456789/170605
Title: Pengaruh Konsentrasi Ekstrak Daun Lemon Verbena terhadap Aktivitas Antibakteri dan Kualitas Mikrobiologi pada Produk Teh
Other Titles: The Effect of Lemon Verbena Leaf Extract Concentration on Antibacterial Activity and Microbiological Quality in Tea Product
Authors: Sarastani, Dewi
FASYA, RAISA
Issue Date: 2025
Publisher: IPB University
Abstract: Penggunaan ekstrak alami dalam produk pangan semakin meningkat seiring dengan kesadaran konsumen akan kesehatan dan keamanan pangan. Penelitian ini bertujuan untuk mengkaji pengaruh konsentrasi ekstrak daun lemon verbena (Aloysia citrodora) terhadap aktivitas antibakteri dan kualitas mikrobiologis pada produk teh. Ekstraksi dilakukan dengan metode maserasi menggunakan etanol, diikuti dengan uji aktivitas antibakteri menggunakan metode difusi cakram terhadap Escherichia coli pada konsentrasi 75%, 50%, dan 25%. Hasil menunjukkan bahwa konsentrasi 75% menghasilkan zona inhibisi terbesar (13,5 mm), dengan analisis regresi menunjukkan hubungan linier yang kuat antara konsentrasi dan efektivitas antibakteri (R² = 0,9868). Selain itu, penambahan ekstrak meningkatkan kualitas mikrobiologis produk teh, dengan nilai Angka Lempeng Total (ALT) memenuhi batas maksimum, sementara produk tanpa ekstrak melebihi batas tersebut. Temuan ini mengonfirmasi potensi ekstrak daun lemon verbena sebagai pengawet alami yang efektif, mampu menghambat pertumbuhan mikroba serta meningkatkan keamanan dan kualitasnya.
The use of natural extracts in food products is increasing in line with consumer awareness of food health and safety. This study aims to examine the effect of lemon verbena leaf extract (Aloysia citrodora) concentration on antibacterial activity and microbiological quality in tea products. Extraction was performed using the maceration method with ethanol, followed by antibacterial activity testing using the disk diffusion method against Escherichia coli at concentrations of 75%, 50%, and 25%. The results showed that the 75% concentration produced the largest inhibition zone (13.5 mm), with regression analysis indicating a strong linear relationship between concentration and antibacterial efficacy (R² = 0.9868). Additionally, the addition of the extract improved the microbiological quality of the tea product, with Total Plate Count (TPC) values meeting the maximum limit, while the product without extract exceeded this limit. These findings confirm the potential of lemon verbena leaf extract as an effective natural preservative, capable of inhibiting microbial growth and enhancing safety and quality.
URI: http://repository.ipb.ac.id/handle/123456789/170605
Appears in Collections:UT - Supervisor of Food Quality Assurance

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