Pengaruh Enzim ß-mannanase pada Media Budidaya Larva Black Soldier Fly (Hermetia illucens) terhadap Performa dan Kandungan Kitin

Abstract

Penelitian ini bertujuan menganalisis pengaruh pemberian enzim ß-mannanase pada media palm kernel meal (PKM) terhadap performa dan kandungan kitin larva BSF yang dibudidaya sebagai pakan. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 3 perlakuan dan 5 ulangan. Perlakuan yang digunakan adalah P1 (Media PKM), P2 (Media PKM + enzim ß-mannanase 8000 ppm), dan P3 (Media PKM + enzim ß-mannanase 8000 ppm yang dihidrolisis selama 3 hari). Parameter terdiri dari performa larva BSF, yaitu Total Harvested Larvae (THL), Individual Larvae Weight (ILW), Feed Conversion Ratio (FCR), Survival Rate (SR), panjang dan lebar larva BSF, Income Over Feed Cost (IOFC), serta rendemen kitin. Hasil penelitian menunjukkan bahwa P3 berpengaruh signifikan (P<0,05) terhadap tingginya THL, ILW, panjang, lebar, IOFC, dan rendahnya FCR. Hasil penelitian menunjukkan tidak ada perbedaan signifikan (P>0,05) terhadap rendemen kitin. Simpulan penelitian ini adalah kombinasi media PKM dan enzim ß-mannanase 8000 ppm yang dihidrolisis selama 3 hari (P3) dapat menghasilkan larva berkualitas tinggi yang potensial sebagai pakan.

This study aims to analyze the effect of adding ß-mannanase enzyme to palm kernel meal (PKM) medium on the performance and chitin content of BSF larvae cultivated as feed. This study used a completely randomized design (CRD) with 3 treatments and 5 replicates. The treatments used were P1 (PKM medium), P2 (PKM medium + ß-mannanase enzyme at 8000 ppm), and P3 (PKM medium + ß-mannanase enzyme at 8000 ppm hydrolyzed for 3 days). The parameters included BSF larvae performance, namely Total Harvested Larvae (THL), Individual Larvae Weight (ILW), Feed Conversion Ratio (FCR), Survival Rate (SR), BSF larvae length and width, Income Over Feed Cost (IOFC), and chitin yield. The results showed that P3 significantly influenced (P<0.05) higher THL, ILW, length, width, IOFC, and lower FCR. The results indicated no significant difference (P>0.05) in chitin yield. The conclusion of this study is that the combination of PKM medium and 8000 ppm ß-mannanase enzyme hydrolyzed for 3 days (P3) can produce high-quality larvae that are potential feed.